Abstract

Volume.119 Number.8

Original article : Basic science

Antimicrobial Effects of Iodine-Polyvinyl Alcohol Ophthalmic and Eye Washing Solution (PA・IODO) with Special Reference to its Temperature, Concentration and Time and its Preservation Stability
Hiroshi Hatano1, Masako Sakamoto2, Kazuo Hayashi3, Seigo Kamiya3
1 Lumine Hatano Eye Clinic
2 The Research Foundation for Microbial Diseases Osaka University
3 Nitten Pharmaceutical Co., Ltd.

Purpose: Temperature, concentration and time are the three factors that affect the inactivation capacity of iodine antiseptics. We investigated the effect of these factors on the microbe inactivation of Iodine-Polyvinyl Alcohol ophthalmic and eye washing solution (PA・IODO), and also investigated the preservation conditions on stability of the inactivation activity of the PA・IODO.
Methods: Test microbes were mixed with PA・IODO, varying the three factors. The live microbes were counted after each reaction. The effects of plugging and preservation temperature were investigated to determine the preserving stability.
Results: The inactivation capacity of PA・IODO tended to decrease in almost all microbes tested at 4°C. Twenty times or less diluted PA・IODO killed almost all microbes completely. The time effect was more marked in viruses. Plugging and low-temperature made iodine concentration in diluted PA・IODO remain relatively high.
Conclusions: The concentration of PA・IODO affected the inactivation ability more than the temperature and time, although all the three factors correlated positively to the inactivation. For preservation the diluted PA・IODO needed plugging and low temperature.
Nippon Ganka Gakkai Zasshi (J Jpn Ophthalmol Soc) 119: 503-510, 2015.

Key words
Iodine antiseptic, Microbial inactivation, Drug-resistant microorganisms, Preservation stability
Reprint requests to
Hiroshi Hatano, M.D. Lumine Hatano Eye Clinic. 438-1 Fujisawa, Fujisawa-shi, Kanagawa-ken 251-0052, Japan